Azopyram kit, ready-made solution, 200 ml

Indications for use

The kit is used to control the thoroughness of pre-sterilization cleaning of all medical instruments that come into contact with the wound surface, come into contact with blood or injectable drugs (for example, a set of operating instruments).

Azopyram-Kit is used on medical products to detect traces of:

• blood;
• rust;
• washing powder with bleaches;
• acids;
• oxidizing agents (in particular, chrome mixture, bleach, chloramine, etc.);
• peroxidases of plant origin.

Inspections are carried out at disinfection and sanitary-epidemiological stations, as well as in all medical and preventive institutions.

basic information

The azopyram test is an assessment of the quality of disinfection and pre-sterilization treatment. It is carried out before the sterilization procedure for each batch of instruments processed during the day.

The azopyram test allows you to determine whether biological material remains on the instruments - blood, skin particles, biological secretions. The requirement to periodically assess the quality of pre-sterilization cleaning is regulated by SanPiN 2.1.3.2630-10 “Sanitary and epidemiological requirements for institutions engaged in medical activities.”

Disinfection and pre-sterilization cleaning of manicure instruments: how to choose a disinfectant and prepare the solution correctly

Paragraph 2.14 of the document describes how often you need to do an azopyram test and what to do with the results:

• How often should I do an azopyram test? Quality control is carried out daily;
• How many instruments are tested? 1% from each batch of processed instruments, but not less than 3 pcs. If you have to process 20 manicure tools, then you need to take 3 of them for testing;
• What to do with the results? The result is entered into the journal “Accounting for the quality of pre-sterilization treatment”. The correctness and systematic filling of the log is checked by Rospotrebnadzor during scheduled and unscheduled inspections. In this case, only negative results should be recorded in the journal. If positive, disinfection and PSO are repeated to eliminate contamination.

Stages of implementation:

1. Preparation of the solution;
2. Carrying out a test;
3. Analysis of results and recording in the log book.

Safety precautions:

• the procedure should be carried out wearing gloves, goggles and a protective mask. Aniline, one of the components of azomiram liquid, is a toxic substance and can lead to poisoning;
• the room where the azopyram test is carried out is ventilated after the procedure so that toxic substances do not linger in the air.

Directions for use and dosage

To prepare the working composition, you need to transfer aniline hydrochloride (10 ml) into a bottle with amidopyrine solution (90 ml) and mix thoroughly. Immediately before the pre-sterilization test, the solution should be brought to a volume of 200 ml by adding 100 ml of 3% (pharmacy) hydrogen peroxide. The resulting mixture must be thoroughly mixed until completely dissolved and used within 2 hours.

Before starting work, you should conduct a suitability test, for which 2-3 drops of the working solution are applied to the blood stain. If a violet color appears within 1 minute, the reagent is considered suitable for use. If staining does not occur or it appears 1 minute after the test, the result is not taken into account and this solution cannot be used. Monitoring the cleaning of medical instruments before sterilization using Azopyram-Kit is carried out in medical institutions at least once a week . The control process is organized and carried out by the head nurse of the department. Once a quarter, control is carried out by sanitary and epidemiological stations.

The working solution (Azopyram-Kit reagents and 3% hydrogen peroxide in a 1:1 ratio) is applied to the medical instruments and products under study - wiped with a swab or applied directly to the instrument using a pipette. To check syringes (reusable), pour 3-4 drops of the working solution into them and move the piston several times to wet the entire inner surface of the instrument. To assess the quality of cleaning of catheters and other hollow products, a solution is injected into them using a syringe or pipette. The solution is left for 1 minute, after which it is poured onto a gauze napkin. The amount of reagent required to test a product directly depends on its size.

Using one Azopyram-Kit, you can carry out about 2000 reactions, based on the consumption of the working solution: 2 drops per 1 test.

Azopyram (1-fl.-10 g)

Azopyram is a reagent for detecting hidden traces of blood on medical instruments that have been prepared for sterilization. This drug is used to control the thoroughness of pre-sterilization cleaning of all medical instruments that come into contact with the wound surface, come into contact with blood or injectable drugs (for example, a set of operating instruments). In addition to traces of blood, Azopyram is used to detect traces of rust, washing powder with bleaches, and acids on medical products. Trade name "Azopyram-set". The composition of the Azopyram reagent includes amidopyrine 10% (in ethyl alcohol solution), aniline hydrochloride 0.10-0.15%. The Azopyram kit includes a package of reagent 1, reagent 2 in an orange test tube and a white test tube with reagent 3. Using 1 set of Azopyram, you can carry out about 2000 reactions (if the consumption of the working solution is 2 drops per reaction). Application of the Azopyram reagent To prepare 1 liter of solution, 100 g of amidopyrine and 1.0-1.5 g of aniline hydrochloride are required. The solution must be brought to a volume of 1 liter with the addition of 95% ethyl alcohol. The resulting mixture must be thoroughly mixed until completely dissolved. To directly check the quality of cleaning of medical instruments, it is necessary to prepare a working solution by mixing equal parts of Azopyram and a 3% hydrogen peroxide solution. Before use, you should test the suitability of the drug; for this, 2-3 drops of the working solution should be applied to the blood stain. If a violet color appears within 1 minute, the reagent is considered suitable for use. If coloring does not appear, then the solution cannot be used. Coloring that appears 1 minute after the test is not taken into account. Monitoring the cleaning of medical instruments before sterilization using the Azopyram-kit is carried out in medical institutions at least once a week. The control process is organized and carried out by the head nurse of the department. Once a quarter, control is carried out by sanitary and epidemiological stations. Instructions for use of Azopyram The working solution (Azopyram and 3% hydrogen peroxide in a 1:1 ratio) is applied to the medical instruments and products under study - wiped with a swab or applied directly to the instrument using a pipette. To check syringes (reusable), pour 3-4 drops of the working solution into them and move the piston several times to wet the entire inner surface of the instrument. To assess the quality of cleaning of catheters and other hollow products, Azopyram is injected into them using a syringe or pipette. The solution is left for 1 minute, after which it is poured onto a gauze napkin. The amount of reagent required to test a product directly depends on its size. Storage of the drug The instructions for Azopyram state that the shelf life of the prepared solution in a tightly closed dark bottle at a temperature of +4 C (that is, in the refrigerator) is 2 months. If the solution is stored in the dark, but at room temperature, then its shelf life is no more than 1 month. Features of using Azopyram In the case of moderate yellowing of the reagent, Azopyram (without precipitation) does not reduce its quality in any way. When checking medical instruments, they should be at room temperature. Hot products should not be tested, and the solution should not be used near heating devices. The working solution (Azopyram + 3% hydrogen peroxide) should not be kept in bright light and can only be used for 2 hours.

Please check with the seller for detailed information about the product by contacting him at the phone number indicated above or by sending an electronic request through the feedback form.

special instructions

Moderate yellowing of the kit reagents (without precipitation) does not in any way reduce its quality.

When checking medical instruments, they must be at room temperature. Do not test hot products, and do not use the solution near heating devices.

The working solution (Azopyram-Kit reagents + 3% hydrogen peroxide) should not be kept in bright light and can only be used for 2 hours.

The drug is able to retain its properties after freezing and subsequent thawing.

Preparation of the solution

There are two ways to prepare the liquid for the procedure:

• from amidopyrine in the form of a dry powder. For 1 liter of the finished reagent, mix 100 g of amidopyrine in a clean, dry container with 1-1.5 g of aniline hydrochloride. Bring the mixture to 1 liter by adding 95% ethyl alcohol;
• from an azopyram kit, including 2 mixing reagents. To prepare, simply mix the contents of two bottles thoroughly. There is such a kit.

The resulting solution is ready, but not working. To prepare the working fluid, it is necessary to add hydrogen peroxide to azopyram.

1. Measure out an equal amount of prepared azopyram solution and 3% hydrogen peroxide;
2. Mix azopyram and peroxide and you will get a working reagent.

The working solution must be used no later than 60-120 minutes after preparation. After this time, it is unsuitable for work.

For the exact service life of the working fluid, see the instructions for the specific product.

The stores offer different kits and reagents for preparing the solution, including those with letter additives:

• Azopyram-D;
• Azopyram-R;
• Azopyram-SK;
• Azopyram-P;
• Azopyram-K.

Depending on the product you choose, the technology for preparing the finished solution will change slightly, so be sure to study the instructions for the drug. In this case, the evaluation of the solution and the test result do not depend on the manufacturer and kit configuration.

Shelf life of Azopyram

Different release forms have different shelf life:

• Powder concentrate (Azopyram SK) – stored for 2 years.
• Alcohol solution of reagents (Azopyram Kit) - no longer than 2 months.

How to identify an expired or defective drug?

In the case of a powder product, the defect can only be determined by the damaged packaging, and the product itself remains unchanged. Therefore, you should carefully read the information on the packaging.

The alcohol solution has a characteristic odor. If it is absent, it is difficult to consider the drug spoiled.

What happens to the product over time?

Over time, powdered Azopyram does not change its appearance, but the components lose their properties.

As for the solution, an important change, in addition to the loss of properties, is the absence of odor or its weakening. Occasionally, sediment may appear.

Checking the working solution

If you are not sure whether the prepared liquid can be used, do the test:

1. Apply a few drops of the solution to the blood stain using a pipette;
2. After exactly 1 minute, check the color of the liquid. If it turns out purple-lilac, you can use the product. If a pronounced color does not appear at all, the product is not suitable for use.

In the absence of blood, a performance test can be performed by dropping an azopyram solution onto a drop of detergent. In this case, the suitable liquid will turn pink.

The prepared solution, not diluted with hydrogen peroxide, and poured into a bottle with a tight lid can be stored for no more than 1 month at room temperature and no more than two months at temperatures below +4 degrees. The storage place should be dark.

To control storage conditions, the mixing date is marked on the bottle with the finished liquid.

Carrying out an azopyram test

Features of the event:

1. The instrument must be dry and at room temperature. If moisture particles remain on the surface, wipe the devices thoroughly with a dry cloth;
2. Place the tools in 1 layer on a clean napkin;
3. Pipette a drop of the prepared azopyram solution onto all cutting parts, as well as into the joints and handles;
4. Wait exactly 1 minute and evaluate the result.

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